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| NA0800 - Bulletin (1MB) |
| Similar Products |
NA0800
Sigma
GenElute™ HP Select Plasmid Gigaprep Kit
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Description
| Features and Benefits | • Endotoxin-free plasmid recoveries of up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg • Only 90 minutes from pelleted cells to purified plasmid • Yields high-quality plasmid DNA necessary for high transfection efficiency in endotoxin-sensitive eukaryotic cell lines |
| General description | The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost-effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤ 0.1 Endotoxin Unit/μg can be recovered from E. coli cultures in 1.2-2.5 liters Luria Broth (LB) medium or 600 mL - 1.2 liters of Terrific Broth (TB) medium in less than 2.0 hours after harvesting the cells. An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts,1, 2 while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominantly in its supercoiled form, and it is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR,† transformation, and transfection. Genomic DNA and RNA are below detectable levels as determined by ethidium bromide-stained agarose gel electrophoresis. |
| Other Notes | For additional information, please see www.sigma-aldrich.com/genelutehp. |
| Legal Information | GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| usage | sufficient for 5 preparations |
| storage temp. | 20-25°C |
Safety
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/37/38-48/20-67 |
| Safety Statements | 53-26-36/37/39 |
| RIDADR | UN 1993 9/PG 3 |
